Allis (Alosa alosa) and Twaite (Alosa fallax) shad monitoring data sampled from the rivers Wye, Usk and Tywi using Mitochondrial DNA techniques (2013).

The river Wye, Usk and Tywi are, or form part of Special Areas of Conservation (SACs), designed under the EU Habitats Directive (92/43/EEC) for several features including the Twaite and Allis shad. The dataset contains the results from DNA analyses conducted on 226 fish eggs sampled from the river Wye, Usk and Tywi. The aim of the project was to use genetic techniques to address the weaknesses identified in the methods already adopted by Natural Resources Wales (NRW) for sampling shad eggs. The main objectives of analyses were to: - Determine whether the collected eggs belonged to Alosa spp. - Determine the proportion of Twaite and Allis shad using three different genetic markers.

Identification

Identifier
NRW_DS115834
Alternative Title
  • Monitoring Allis and Twaite Shad: Quality assurance and species Identification using molecular techniques
  • Data monitro Herlynod (Alosa alosa) a Gwangod (Alosa fallax) a samplwyd o afonydd Gwy, Wysg a Tywi, gan ddefnyddio technegau DNA Mitocondriaidd (2013).
Metadata Language
English
Lineage

Analyses of eggs collected involved DNA barcoding where a short genetic marker, usually the COI gene, of an unknown organism was sequenced using universal primers, to identify the species to which it belongs. This DNA analyses was completed in response to being unable to distinguish between the two Alosa species and reduce the risk of miss identification of fish eggs.
Additional Information

Relevant NRW Evidence Report: No 001 Monitoring Allis and Twaite shad: Quality assurance and species identification using molecular techniques (2013) Hardouin, E. Andreou, D. Stuart, S. Natural Resources Wales Report. Located on the DMS: KNOW-101-3
Dataset Reference Date (Publication)
2013-11-22
Owner
  Natural Resources Wales (NRW)
Maes-y-Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW, Wales
http://naturalresources.wales/?lang=en
Originator
  Bournemouth University
Bournemouth University, Fern Barrow, Talbot Campus, Poole, Dorset, BH12 5BB, UK
https://www1.bournemouth.ac.uk/
 

Extent

Extent

Geographic Extent
POWYS - POWYS
Geographic Extent
SIR FYNWY - MONMOUTHSHIRE
Geographic Extent
SIR GAERFYRDDIN - CARMARTHENSHIRE
N
W
E
S
Map representing the geographic extent of the dataset


 
 

Distribution

Format Type and Description
  • Documents ()

    Specification

    [A] The final report in Microsoft Word and Adobe PDF formats. [B] Electronic copies of the field sheets, saved as pdfs. [C] An Excel spreadsheet Ninf_mito_microsat_scoring detailing the RFLP, mtDNA and microsatellite results for each egg. [D] Three FASTA format files, TreeUniveralPrimers2.fas; Ninf_sequences.fas and Shad_CytochromeB_all2.fas.

 

Quality

Data quality

Quality Scope
Dataset
Conformity
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
Explanation

This dataset has not been assessed for conformance to the referenced INSPIRE regulation.
Degree
false
Lineage

Analyses of eggs collected involved DNA barcoding where a short genetic marker, usually the COI gene, of an unknown organism was sequenced using universal primers, to identify the species to which it belongs. This DNA analyses was completed in response to being unable to distinguish between the two Alosa species and reduce the risk of miss identification of fish eggs.
 

Keywords

Keywords

GEMET - INSPIRE themes, version 1.0
Type
Theme

Citation

Date (Publication)
2008-06-01
Code
geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme

Keywords

NRW Thesaurus
  • freshwater fish
  • twaite shad
  • alosa fallax
  • allis shad
  • alosa alosa
  • River Usk
  • molecular ecology
Type
Theme

Citation

Date (Publication)
2023-12-31
Code
geonetwork.thesaurus.local.theme.converted_nrw-keywords
 

Spatial Reference System

Code
British National Grid (EPSG::27700)
 

Content

Content Information

NRW Profile

Custom Elements

NRW Related Title

Monitoring Allis and Twaite Shad: quality assurance and species identification using molecular techniques 2013
 

Constraints

Constraints

Limitations on Public Access and Use

Restriction type
Other restrictions
 

Access Constraints Directive

Limitations
no limitations
 

Access Constraints Text

Other constraints

There are no access restrictions to this data. NRW may release, publish or disseminate it freely.
 

Use Constraints

Use constraints type
Other restrictions
Other constraints

© CNC/NRW, 2014 There are no use restrictions on this data. Recipients may re-use, reproduce, disseminate this data free of charge in any format or medium, provided they do so accurately, acknowledging both the source and NRW's copyright, and do not use it in a misleading context. It is the recipient's responsibility to ensure the data is fit for the intended purpose, that dissemination or publishing does not result in duplication, and that it is fairly interpreted. Advice on interpretation should be sought where required. To avoid re-using old data, users should periodically obtain the latest version from the original source.
 
 
 

Metadata

Metadata

File Identifier
4f4c4942-4343-5764-6473-313135383334 XML
Metadata Language
English
Resource type
Dataset
Metadata Date
2025-03-07T17:14:15.975Z
Metadata Standard Name
NRW
Metadata Standard Version

1.0

pointOfContact
  Natural Resources Wales (NRW)
Maes-y-Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW, Wales
http://naturalresources.wales/?lang=en
 
 

Publishing Body

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