Allis (Alosa alosa) and Twaite (Alosa fallax) shad monitoring data sampled from the rivers Wye, Usk and Tywi using Mitochondrial DNA techniques (2013).
The river Wye, Usk and Tywi are, or form part of Special Areas of Conservation (SACs), designed under the EU Habitats Directive (92/43/EEC) for several features including the Twaite and Allis shad. The dataset contains the results from DNA analyses conducted on 226 fish eggs sampled from the river Wye, Usk and Tywi. The aim of the project was to use genetic techniques to address the weaknesses identified in the methods already adopted by Natural Resources Wales (NRW) for sampling shad eggs. The main objectives of analyses were to: - Determine whether the collected eggs belonged to Alosa spp. - Determine the proportion of Twaite and Allis shad using three different genetic markers.
- Identification
- Extent
- Distribution
- Quality
- Keywords
- Spatial Reference System
- Content
- Constraints
- Maintenance
- Metadata
Identification
- Identifier
- NRW_DS115834
- Alternative Title
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- Monitoring Allis and Twaite Shad: Quality assurance and species Identification using molecular techniques
- Data monitro Herlynod (Alosa alosa) a Gwangod (Alosa fallax) a samplwyd o afonydd Gwy, Wysg a Tywi, gan ddefnyddio technegau DNA Mitocondriaidd (2013).
- Metadata Language
- English
- Lineage
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Analyses of eggs collected involved DNA barcoding where a short genetic marker, usually the COI gene, of an unknown organism was sequenced using universal primers, to identify the species to which it belongs. This DNA analyses was completed in response to being unable to distinguish between the two Alosa species and reduce the risk of miss identification of fish eggs.
- Additional Information
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Relevant NRW Evidence Report: No 001 Monitoring Allis and Twaite shad: Quality assurance and species identification using molecular techniques (2013) Hardouin, E. Andreou, D. Stuart, S. Natural Resources Wales Report. Located on the DMS: KNOW-101-3
- Dataset Reference Date (Publication)
- 2013-11-22
Temporal Extent
- Begin date
- 2013-06-04
- End date
- 2013-06-13
- Topic category
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- Biota
Extent
Extent
- Geographic Extent
- POWYS - POWYS
- Geographic Extent
- SIR FYNWY - MONMOUTHSHIRE
- Geographic Extent
- SIR GAERFYRDDIN - CARMARTHENSHIRE
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Distribution
- Format Type and Description
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Documents
Documents
()
- Specification
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[A] The final report in Microsoft Word and Adobe PDF formats. [B] Electronic copies of the field sheets, saved as pdfs. [C] An Excel spreadsheet Ninf_mito_microsat_scoring detailing the RFLP, mtDNA and microsatellite results for each egg. [D] Three FASTA format files, TreeUniveralPrimers2.fas; Ninf_sequences.fas and Shad_CytochromeB_all2.fas.
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Documents
Documents
()
Quality
Data quality
- Quality Scope
- Dataset
- Lineage
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Analyses of eggs collected involved DNA barcoding where a short genetic marker, usually the COI gene, of an unknown organism was sequenced using universal primers, to identify the species to which it belongs. This DNA analyses was completed in response to being unable to distinguish between the two Alosa species and reduce the risk of miss identification of fish eggs.
Keywords
Keywords
- Type
- Theme
Citation
- Date (Publication)
- 2008-06-01
Keywords
- NRW Thesaurus
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- freshwater fish
- twaite shad
- alosa fallax
- allis shad
- alosa alosa
- River Usk
- molecular ecology
- Type
- Theme
Citation
- Date (Publication)
- 2023-12-31
Spatial Reference System
Content
Content Information
NRW Profile
Custom Elements
- NRW Related Title
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Monitoring Allis and Twaite Shad: quality assurance and species identification using molecular techniques 2013
Constraints
Constraints
Limitations on Public Access and Use
- Restriction type
- Other restrictions
Access Constraints Directive
- Limitations
- no limitations
Access Constraints Text
- Other constraints
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There are no access restrictions to this data. NRW may release, publish or disseminate it freely.
Use Constraints
- Use constraints type
- Other restrictions
- Other constraints
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© CNC/NRW, 2014 There are no use restrictions on this data. Recipients may re-use, reproduce, disseminate this data free of charge in any format or medium, provided they do so accurately, acknowledging both the source and NRW's copyright, and do not use it in a misleading context. It is the recipient's responsibility to ensure the data is fit for the intended purpose, that dissemination or publishing does not result in duplication, and that it is fairly interpreted. Advice on interpretation should be sought where required. To avoid re-using old data, users should periodically obtain the latest version from the original source.
Metadata
Metadata
- File Identifier
- 4f4c4942-4343-5764-6473-313135383334 XML
- Metadata Language
- English
- Resource type
- Dataset
- Metadata Date
- 2024-05-31T09:37:53.961Z
- Metadata Standard Name
- NRW
- Metadata Standard Version
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1.0